Improving oral implant osseointegration in a murine model via Wnt signal amplification
Identifieur interne : 000045 ( France/Analysis ); précédent : 000044; suivant : 000046Improving oral implant osseointegration in a murine model via Wnt signal amplification
Auteurs : Sylvain Mouraret [États-Unis, France] ; Daniel J. Hunter [États-Unis] ; Claire Bardet [États-Unis, France] ; Antoine Popelut [France] ; John B. Brunski [États-Unis] ; Catherine Chaussain [France] ; Philippe Bouchard [France] ; Jill A. Helms [États-Unis]Source :
- Journal of clinical periodontology [ 0303-6979 ] ; 2013.
Abstract
To determine the key biological events occurring during implant failure and then we use this knowledge to develop new biology-based strategies that improve osseointegration.
Wild-type and
Maxillary implants lacking primary stability show histological evidence of persistent fibrous encapsulation and mobility, which recapitulates the clinical problems of implant failure. Despite histological and molecular evidence of fibrous encapsulation, osteoblasts in the gap interface exhibit robust ALP activity. This mineralization activity is counteracted by osteoclast activity that resorbs any new bony matrix and consequently, the fibrous encapsulation remains. Using a genetic mouse model, we show that implants lacking primary stability undergo osseointegration, provided that Wnt signalling is amplified.
In a mouse model of oral implant failure caused by a lack of primary stability, we find evidence of active mineralization. This mineralization, however, is outpaced by robust bone resorption, which culminates in persistent fibrous encapsulation of the implant. Fibrous encapsulation can be prevented and osseointegration assured if Wnt signalling is elevated at the time of implant placement.
Url:
DOI: 10.1111/jcpe.12187
PubMed: 24164629
PubMed Central: 4993043
Affiliations:
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PMC:4993043Le document en format XML
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Brunski</name><affiliation wicri:level="2"><nlm:aff id="A1">Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Chaussain, Catherine" sort="Chaussain, Catherine" uniqKey="Chaussain C" first="Catherine" last="Chaussain">Catherine Chaussain</name><affiliation wicri:level="1"><nlm:aff id="A3">Dental School University Paris Descartes PRES Sorbonne Paris Cité, Montrouge, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Dental School University Paris Descartes PRES Sorbonne Paris Cité, Montrouge</wicri:regionArea><wicri:noRegion>Montrouge</wicri:noRegion><wicri:noRegion>Montrouge</wicri:noRegion></affiliation></author><author><name sortKey="Bouchard, Philippe" sort="Bouchard, Philippe" uniqKey="Bouchard P" first="Philippe" last="Bouchard">Philippe Bouchard</name><affiliation wicri:level="3"><nlm:aff id="A2">Department of Periodontology, Service of Odontology, Rothschild Hospital, AP-HP, Paris 7 – Denis, Diderot University, U.F.R. of Odontology, Paris, France</nlm:aff><country xml:lang="fr">France</country><wicri:regionArea>Department of Periodontology, Service of Odontology, Rothschild Hospital, AP-HP, Paris 7 – Denis, Diderot University, U.F.R. of Odontology, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Helms, Jill A" sort="Helms, Jill A" uniqKey="Helms J" first="Jill A." last="Helms">Jill A. Helms</name><affiliation wicri:level="2"><nlm:aff id="A1">Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford, CA</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author></analytic><series><title level="j">Journal of clinical periodontology</title><idno type="ISSN">0303-6979</idno><idno type="eISSN">1600-051X</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="S1"><title>Aim</title><p id="P1">To determine the key biological events occurring during implant failure and then we use this knowledge to develop new biology-based strategies that improve osseointegration.</p></sec><sec id="S2"><title>Materials and Methods</title><p id="P2">Wild-type and <italic>Axin2<sup>LacZ/LacZ</sup></italic> adult male mice underwent oral implant placement, with and without primary stability. Peri-implant tissues were evaluated using histology, alkaline phosphatase (ALP) activity, tartrate resistant acid phosphatase (TRAP) activity and TUNEL staining. In addition, mineralization sites, collagenous matrix organization and the expression of bone markers in the peri-implant tissues were assessed.</p></sec><sec id="S3"><title>Results</title><p id="P3">Maxillary implants lacking primary stability show histological evidence of persistent fibrous encapsulation and mobility, which recapitulates the clinical problems of implant failure. Despite histological and molecular evidence of fibrous encapsulation, osteoblasts in the gap interface exhibit robust ALP activity. This mineralization activity is counteracted by osteoclast activity that resorbs any new bony matrix and consequently, the fibrous encapsulation remains. Using a genetic mouse model, we show that implants lacking primary stability undergo osseointegration, provided that Wnt signalling is amplified.</p></sec><sec id="S4"><title>Conclusions</title><p id="P4">In a mouse model of oral implant failure caused by a lack of primary stability, we find evidence of active mineralization. This mineralization, however, is outpaced by robust bone resorption, which culminates in persistent fibrous encapsulation of the implant. Fibrous encapsulation can be prevented and osseointegration assured if Wnt signalling is elevated at the time of implant placement.</p></sec></div></front></TEI><affiliations><list><country><li>France</li><li>États-Unis</li></country><region><li>Californie</li><li>Île-de-France</li></region><settlement><li>Paris</li></settlement></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Mouraret, Sylvain" sort="Mouraret, Sylvain" uniqKey="Mouraret S" first="Sylvain" last="Mouraret">Sylvain Mouraret</name></region><name sortKey="Bardet, Claire" sort="Bardet, Claire" uniqKey="Bardet C" first="Claire" last="Bardet">Claire Bardet</name><name sortKey="Brunski, John B" sort="Brunski, John B" uniqKey="Brunski J" first="John B." last="Brunski">John B. Brunski</name><name sortKey="Helms, Jill A" sort="Helms, Jill A" uniqKey="Helms J" first="Jill A." last="Helms">Jill A. Helms</name><name sortKey="Hunter, Daniel J" sort="Hunter, Daniel J" uniqKey="Hunter D" first="Daniel J." last="Hunter">Daniel J. Hunter</name></country><country name="France"><region name="Île-de-France"><name sortKey="Mouraret, Sylvain" sort="Mouraret, Sylvain" uniqKey="Mouraret S" first="Sylvain" last="Mouraret">Sylvain Mouraret</name></region><name sortKey="Bardet, Claire" sort="Bardet, Claire" uniqKey="Bardet C" first="Claire" last="Bardet">Claire Bardet</name><name sortKey="Bouchard, Philippe" sort="Bouchard, Philippe" uniqKey="Bouchard P" first="Philippe" last="Bouchard">Philippe Bouchard</name><name sortKey="Chaussain, Catherine" sort="Chaussain, Catherine" uniqKey="Chaussain C" first="Catherine" last="Chaussain">Catherine Chaussain</name><name sortKey="Popelut, Antoine" sort="Popelut, Antoine" uniqKey="Popelut A" first="Antoine" last="Popelut">Antoine Popelut</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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